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NDB ID: PH0037    PDB ID: 2O5J 


Title:

CRYSTAL STRUCTURE OF THE T. THERMOPHILUS RNAP POLYMERASE ELONGATION COMPLEX WITH THE NTP SUBSTRATE ANALOG

Molecular Description:

DNA-directed RNA polymerase alpha chain
DNA-directed RNA polymerase beta chain
DNA-directed RNA polymerase beta' chain
DNA-directed RNA polymerase omega chain (E.C.2.7.7.6)/DNA/RNA Complex

Structural Keywords:

RH DOUBLE HELIX, OVERHANGING BASES, NICKED

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 4 protein sequences

Primary Citation:

Vassylyev, D.G., Vassylyeva, M.N., Zhang, J., Palangat, M., Artsimovitch, I., Landick, R.
Structural basis for substrate loading in bacterial RNA polymerase. 
Nature, 448, pp. 163 - 168, 2007.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 41

Cell Constants:

a = 152.34   b = 152.34   c = 524.567 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS 1.1 program. The R value is 0.2293 for 199490 reflections in the resolution range 40.0 to 3.0 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)