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NDB ID: PH0049    PDB ID: 3E3J 


Title:

CRYSTAL STRUCTURE OF AN INTERMEDIATE COMPLEX OF T7 RNAP AND 8NT OF RNA

Molecular Description:

DNA-directed RNA polymerase (E.C.2.7.7.6)/RNA/DNA complex

Structural Keywords:

RH DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Durniak, K.J., Bailey, S., Steitz, T.A.
The structure of a transcribing t7 RNA polymerase in transition from initiation to elongation 
Science, 322, pp. 553 - 557, 2008.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 99.216   b = 180.602   c = 198.619 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC 5.4.0077 program. The R value is 0.39486 for 5736 reflections in the resolution range 49.81 to 6.7 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)