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NDB ID: PH0054    PDB ID: 3GTJ 


Title:

BACKTRACKED RNA POLYMERASE II COMPLEX WITH 13MER RNA

Molecular Description:

DNA-directed RNA polymerase II subunit RPB1 (E.C.2.7.7.6)
DNA-directed RNA polymerase II subunit RPB2 (E.C.2.7.7.6)
DNA-directed RNA polymerase II subunit RPB3
DNA-directed RNA polymerases I
II
and III subunit RPABC1
DNA-directed RNA polymerases I
II
and III subunit RPABC2
DNA-directed RNA polymerases I
II
and III subunit RPABC3
DNA-directed RNA polymerase II subunit RPB9
DNA-directed RNA polymerases I
II
and III subunit RPABC5
DNA-directed RNA polymerase II subunit RPB11
DNA-directed RNA polymerases I
II
and III subunit RPABC4

Structural Keywords:

B DOUBLE HELIX, OPEN HELIX, MISMATCHED BASE PAIRS

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 10 protein sequences

Primary Citation:

Wang, D., Bushnell, D.A., Huang, X., Westover, K.D., Levitt, M., Kornberg, R.D.
Structural basis of transcription: backtracked RNA polymerase II at 3.4 angstrom resolution. 
Science, 324, pp. 1203 - 1206, 2009.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 170.916   b = 225.5   c = 195.932 (Ångstroms)

α = 90.0   β = 101.21   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC 5.2.0019 program. The R value is 0.28217 for 85572 reflections in the resolution range 50.0 to 3.42 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)