A Portal for Three-dimensional Structural Information about Nucleic Acids
As of 17-Aug-2016 number of released structures: 8433
Search for released structures

NDB ID: PH0057    PDB ID: 3GTM 


Title:

CO-COMPLEX OF BACKTRACKED RNA POLYMERASE II WITH TFIIS

Molecular Description:

DNA-directed RNA polymerase II subunit RPB1 (E.C.2.7.7.6)
DNA-directed RNA polymerase II subunit RPB2 (E.C.2.7.7.6)
DNA-directed RNA polymerase II subunit RPB3
DNA-directed RNA polymerases I
II
and III subunit RPABC1
DNA-directed RNA polymerases I
II
and III subunit RPABC2
DNA-directed RNA polymerases I
II
and III subunit RPABC3
DNA-directed RNA polymerase II subunit RPB9
DNA-directed RNA polymerases I
II
and III subunit RPABC5
DNA-directed RNA polymerase II subunit RPB11
DNA-directed RNA polymerases I
II
and III subunit RPABC4
Transcription elongation factor S-II

Structural Keywords:

B DOUBLE HELIX, OPEN HELIX, MISMATCHED BASE PAIRS

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 11 protein sequences

Primary Citation:

Wang, D., Bushnell, D.A., Huang, X., Westover, K.D., Levitt, M., Kornberg, R.D.
Structural basis of transcription: backtracked RNA polymerase II at 3.4 angstrom resolution. 
Science, 324, pp. 1203 - 1206, 2009.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 172.329   b = 218.53   c = 193.913 (Ångstroms)

α = 90.0   β = 100.32   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.234 for 65617 reflections in the resolution range 40.0 to 3.8 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)