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NDB ID: PR0029    PDB ID: 1GAX 


Title:

CRYSTAL STRUCTURE OF THERMUS THERMOPHILUS VALYL-TRNA SYNTHETASE COMPLEXED WITH TRNA(VAL) AND VALYL-ADENYLATE ANALOGUE

Molecular Description:

VALYL-TRNA SYNTHETASE (E.C.6.1.1.9)/RNA COMPLEX

Structural Keywords:

T SINGLE STRAND

Nucleic Acid Sequence:

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Protein Sequence:

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Primary Citation:

Fukai, S., Nureki, O., Sekine, S., Shimada, A., Tao, J., Vassylyev, D.G., Yokoyama, S.
Structural basis for double-sieve discrimination of L-valine from L-isoleucine and L-threonine by the complex of tRNA(Val) and valyl-tRNA synthetase. 
Cell(Cambridge,Mass.), 103, pp. 793 - 803, 2000.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 42 21 2

Cell Constants:

a = 411.81   b = 411.81   c = 81.97 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.0 for 150205 reflections in the resolution range 30.0 to 2.9 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)