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NDB ID: PR0060    PDB ID: 1K8W 


Title:

CRYSTAL STRUCTURE OF THE E. COLI PSEUDOURIDINE SYNTHASE TRUB BOUND TO A T STEM-LOOP RNA

Molecular Description:

TRNA PSEUDOURIDINE SYNTHASE B (E.C. 4.2.1.70)/RNA Complex

Structural Keywords:

SINGLE STRAND, LOOP, FLIPPED-OUT BASES

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Hoang, C., Ferre-D'Amare, A.R.
Cocrystal structure of a tRNA Psi55 pseudouridine synthase: nucleotide flipping by an RNA-modifying enzyme. 
Cell(Cambridge,Mass.), 107, pp. 929 - 939, 2001.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 145.054   b = 40.361   c = 77.987 (Ångstroms)

α = 90.0   β = 110.6   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS 1.0 program. The R value is 0.184 for 34482 reflections in the resolution range 28.27 to 1.85 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)