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NDB ID: PR0102    PDB ID: 1Q2S 


Title:

CHEMICAL TRAPPING AND CRYSTAL STRUCTURE OF A CATALYTIC TRNA GUANINE TRANSGLYCOSYLASE COVALENT INTERMEDIATE

Molecular Description:

Queuine tRNA-ribosyltransferase (E.C.2.4.2.29)/RNA complex

Structural Keywords:

SINGLE STRAND, LOOP, HAIRPIN LOOP, BULGES, FLIPPED-OUT BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Xie, W., Liu, X., Huang, R.H.
Chemical trapping and crystal structure of a catalytic tRNA guanine transglycosylase covalent intermediate 
Nat.Struct.Biol., 10, pp. 781 - 788, 2003.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

I 4

Cell Constants:

a = 261.961   b = 261.961   c = 55.549 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS 1.1 program. The R value is 0.0 for 30762 reflections in the resolution range 30.0 to 3.2 Ångstroms with Fobs >  2.0 sigma(Fobs) and with I >  2.0 sigma(I)