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NDB ID: PR0126    PDB ID: 1SZ1 


Title:

MECHANISM OF CCA-ADDING ENZYMES SPECIFICITY REVEALED BY CRYSTAL STRUCTURES OF TERNARY COMPLEXES

Molecular Description:

tRNA nucleotidyltransferase (E.C.2.7.7.25)/RNA Complex

Structural Keywords:

T SINGLE STRAND, INTERNAL LOOP, HAIRPIN LOOP

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Xiong, Y., Steitz, T.A.
Mechanism of transfer RNA maturation by CCA-adding enzyme without using an oligonucleotide template. 
Nature, 430, pp. 640 - 645, 2004.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 3 2 1

Cell Constants:

a = 218.403   b = 218.403   c = 170.263 (Ångstroms)

α = 90.0   β = 90.0   γ = 120.0 (degrees)

Refinement:

The structure was refined using the REFMAC 5.2.0005 program. The R value is 0.36131 for 10111 reflections in the resolution range 44.72 to 6.21 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)