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NDB ID: PR0128    PDB ID: 1TFW 


Title:

HOW CCA IS ADDED TO THE 3' END OF IMMATURE TRNA WITHOUT THE USE OF AN OLIGONUCLEOTIDE TEMPLATE

Molecular Description:

tRNA nucleotidyltransferase(E.C.2.7.7.25)/RNA Complex

Structural Keywords:

A DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 4 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Xiong, Y., Steitz, T.A.
Mechanism of transfer RNA maturation by CCA-adding enzyme without using an oligonucleotide template. 
Nature, 430, pp. 640 - 645, 2004.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 116.641   b = 84.77   c = 135.813 (Ångstroms)

α = 90.0   β = 104.66   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC 5.2.0005 program. The R value is 0.19454 for 122568 reflections in the resolution range 47.7 to 2.2 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)