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NDB ID: PR0146    PDB ID: 1YTU 


Title:

STRUCTURAL BASIS FOR 5'-END-SPECIFIC RECOGNITION OF THE GUIDE RNA STRAND BY THE A. FULGIDUS PIWI PROTEIN

Molecular Description:

hypothetical protein AF1318/RNA Complex

Structural Keywords:

A DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Ma, J.B., Yuan, Y.R., Meister, G., Pei, Y., Tuschl, T., Patel, D.J.
Structural basis for 5'-end-specific recognition of guide RNA by the A. fulgidus Piwi protein. 
Nature, 434, pp. 666 - 670, 2005.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

H 3

Cell Constants:

a = 238.747   b = 238.747   c = 52.042 (Ångstroms)

α = 90.0   β = 90.0   γ = 120.0 (degrees)

Refinement:

The structure was refined using the REFMAC 5.2.0005 program. The R value is 0.20689 for 36055 reflections in the resolution range 50.0 to 2.5 Ångstroms with Fobs >  2.0 sigma(Fobs) and with I >  0.0 sigma(I)