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NDB ID: PR0148    PDB ID: 1YYO 


Title:

CRYSTAL STRUCTURE OF RNASE III MUTANT E110K FROM AQUIFEX AEOLICUS COMPLEXED WITH DOUBLE-STRANDED RNA AT 2.9-ANGSTROM RESOLUTION

Molecular Description:

Ribonuclease III(E.C.3.1.26.3)/RNA

Structural Keywords:

A DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Gan, J., Tropea, J.E., Austin, B.P., Court, D.L., Waugh, D.S., Ji, X.
Intermediate states of ribonuclease III in complex with double-stranded RNA 
Structure, 13, pp. 1435 - 1442, 2005.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 84.486   b = 61.625   c = 131.114 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS 1.0 program. The R value is 0.225 for 14294 reflections in the resolution range 30.0 to 2.9 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)