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NDB ID: PR0160    PDB ID: 1ZL3 


Title:

COUPLING OF ACTIVE SITE MOTIONS AND RNA BINDING

Molecular Description:

CRYSTAL STRUCTURE OF THE E. COLI PSEUDOURIDINE SYNTHASE TRUB MUTANT D48N BOUND TO A T STEM-LOOP RNA

Structural Keywords:

T DOUBLE HELIX, HAIRPIN LOOP

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Hoang, C., Hamilton, C.S., Mueller, E.G., Ferre-D'Amare, A.R.
Precursor complex structure of pseudouridine synthase TruB suggests coupling of active site perturbations to an RNA-sequestering peripheral protein domain 
Protein Sci., 14, pp. 2201 - 2206, 2005.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 145.433   b = 40.753   c = 78.803 (Ångstroms)

α = 90.0   β = 110.61   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS 1.0 program. The R value is 0.202 for 10935 reflections in the resolution range 19.92 to 2.8 Ångstroms with Fobs >  0.3 sigma(Fobs) and with I >  0.0 sigma(I)