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NDB ID: PR0188    PDB ID: 2F8S 


Title:

CRYSTAL STRUCTURE OF AA-AGO WITH EXTERNALLY-BOUND SIRNA

Molecular Description:

Argonaute protein/RNA Complex

Structural Keywords:

A DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Yuan, Y.R., Pei, Y., Chen, H.Y., Tuschl, T., Patel, D.J.
A Potential Protein-RNA Recognition Event along the RISC-Loading Pathway from the Structure of A. aeolicus Argonaute with Externally Bound siRNA. 
Structure, 14, pp. 1557 - 1565, 2006.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 79.697   b = 117.516   c = 98.62 (Ångstroms)

α = 90.0   β = 99.12   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC 5.2.0005 program. The R value is 0.21717 for 32694 reflections in the resolution range 40.0 to 3.0 Ångstroms with Fobs >  2.0 sigma(Fobs) and with I >  0.0 sigma(I)