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NDB ID: PR0192    PDB ID: 2DER 


Title:

COCRYSTAL STRUCTURE OF AN RNA SULFURATION ENZYME MNMA AND TRNA-GLU IN THE INITIAL TRNA BINDING STATE

Molecular Description:

tRNA-specific 2-thiouridylase mnmA(E.C.2.8.1.-)/RNA Complex

Structural Keywords:

T SINGLE STRAND, HAIRPIN LOOP

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Numata, T., Ikeuchi, Y., Fukai, S., Suzuki, T., Nureki, O.
Snapshots of tRNA sulphuration via an adenylated intermediate 
Nature, 442, pp. 419 - 424, 2006.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 225.389   b = 175.845   c = 52.965 (Ångstroms)

α = 90.0   β = 101.62   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS 1.1 program. The R value is 0.219 for 60663 reflections in the resolution range 46.75 to 3.1 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)