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NDB ID: PR0229    PDB ID: 2I82 


Title:

CRYSTAL STRUCTURE OF PSEUDOURIDINE SYNTHASE RLUA: INDIRECT SEQUENCE READOUT THROUGH PROTEIN-INDUCED RNA STRUCTURE

Molecular Description:

Lyase (EC 4.2.1.70)/RNA Complex

Deposited:

2006-08-31

Released:

2006-11-21

Structural Keywords:

SINGLE STRAND, HAIRPIN LOOP

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Hoang, C., Chen, J., Vizthum, C.A., Kandel, J.M., Hamilton, C.S., Mueller, E.G., Ferre-D'Amare, A.R.
Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure 
Mol.Cell, 24, pp. 535 - 545, 2006.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 320.737   b = 51.691   c = 81.233 (Ångstroms)

α = 90.0   β = 90.81   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.234 for 82366 reflections in the resolution range 19.83 to 2.05 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)