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NDB ID: PR0233    PDB ID: 2IY5 


Title:

PHENYLALANYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS COMPLEXED WITH TRNA AND A PHENYLALANYL-ADENYLATE ANALOG

Molecular Description:

PHENYLALANYL-TRNA SYNTHETASE ALPHA CHAIN (E.C.6.1.1.20)
PHENYLALANYL-TRNA SYNTHETASE BETA CHAIN (E.C.6.1.1.20)

Deposited:

2006-07-12

Released:

2006-09-06

Structural Keywords:

T, LOOP, HAIRPIN LOOP

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 2 protein sequences

Primary Citation:

Moor, N., Kotik-Kogan, O., Tworowski, D., Sukhanova, M., Safro, M.
The crystal structure of the ternary complex of phenylalanyl-tRNA synthetase with tRNAPhe and a phenylalanyl-adenylate analogue reveals a conformational switch of the CCA end. 
Biochemistry, 45, pp. 10572 - 10583, 2006.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 32 2 1

Cell Constants:

a = 173.4   b = 173.4   c = 139.4 (Ångstroms)

α = 90.0   β = 90.0   γ = 120.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.238 for 34168 reflections in the resolution range 9.99 to 3.1 Ångstroms with Fobs >  2.5 sigma(Fobs) and with I >  0.0 sigma(I)