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NDB ID: PR0294    PDB ID: 2R8S 


Title:

HIGH RESOLUTION STRUCTURE OF A SPECIFIC SYNTHETIC FAB BOUND TO P4-P6 RNA RIBOZYME DOMAIN

Molecular Description:

PROTEIN/RNA Complex

Structural Keywords:

SINGLE STRAND, LOOPS, HAIRPIN LOOP, FLIPPED-OUT BASES, BULGES

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 2 protein sequences

Primary Citation:

Ye, J.D., Tereshko, V., Frederiksen, J.K., Koide, A., Fellouse, F.A., Sidhu, S.S., Koide, S., Kossiakoff, A.A., Piccirilli, J.A.
Synthetic antibodies for specific recognition and crystallization of structured RNA 
Proc.Natl.Acad.Sci.Usa, 105, pp. 82 - 87, 2008.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 133.085   b = 53.23   c = 160.555 (Ångstroms)

α = 90.0   β = 108.42   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC 5.2 program. The R value is 0.19632 for 77127 reflections in the resolution range 20.0 to 1.95 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)