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NDB ID: PR0329    PDB ID: 3BT7 


Title:

STRUCTURE OF E. COLI 5-METHYLURIDINE METHYLTRANSFERASE TRMA IN COMPLEX WITH 19 NUCLEOTIDE T-ARM ANALOGUE

Molecular Description:

tRNA (uracil-5-)-methyltransferase (E.C.2.1.1.35)/RNA Complex

Structural Keywords:

SINGLE STRAND, HAIRPIN LOOP

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Alian, A., Lee, T.T., Griner, S.L., Stroud, R.M., Finer-Moore, J.
Structure of a TrmA-RNA complex: A consensus RNA fold contributes to substrate selectivity and catalysis in m5U methyltransferases. 
Proc.Natl.Acad.Sci.Usa, 105, pp. 6876 - 6881, 2008.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 184.407   b = 70.131   c = 107.95 (Ångstroms)

α = 90.0   β = 120.88   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC 5.4.0067 program. The R value is 0.2297 for 38764 reflections in the resolution range 35.07 to 2.43 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)