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NDB ID: PR0343    PDB ID: 2ZNI 


Title:

CRYSTAL STRUCTURE OF PYRROLYSYL-TRNA SYNTHETASE-TRNA(PYL) COMPLEX FROM DESULFITOBACTERIUM HAFNIENSE

Molecular Description:

Pyrrolysyl-tRNA synthetase(E.C. 6.1.1.26)/RNA Complex

Structural Keywords:

T SINGLE STRAND, HAIRPIN LOOP

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Nozawa, K., O'Donoghue, P., Gundllapalli, S., Araiso, Y., Ishitani, R., Umehara, T., Soll, D., Nureki, O.
Pyrrolysyl-tRNA synthetase-tRNA(Pyl) structure reveals the molecular basis of orthogonality 
Nature, 457, pp. 1163 - 1167, 2009.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 43 21 2

Cell Constants:

a = 103.627   b = 103.627   c = 214.044 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX (phenix.refine) program. The R value is 0.228 for 21576 reflections in the resolution range 46.636 to 3.1 Ångstroms with Fobs >  1.48 sigma(Fobs) and with I >  0.0 sigma(I)