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NDB ID: PR0349    PDB ID: 3DH3 


Title:

CRYSTAL STRUCTURE OF RLUF IN COMPLEX WITH A 22 NUCLEOTIDE RNA SUBSTRATE

Molecular Description:

Ribosomal large subunit pseudouridine synthase F(E.C.5.4.99.-)/RNA Complex

Structural Keywords:

SINGLE STRAND, HAIRPIN LOOP

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Alian, A., DeGiovanni, A., Griner, S.L., Finer-Moore, J.S., Stroud, R.M.
Crystal structure of an RluF-RNA complex: a base-pair rearrangement is the key to selectivity of RluF for U2604 of the ribosome. 
J.Mol.Biol., 388, pp. 785 - 800, 2009.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 89.552   b = 84.128   c = 91.354 (Ångstroms)

α = 90.0   β = 94.87   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC 5.4.0067 program. The R value is 0.2336 for 25651 reflections in the resolution range 49.51 to 3.0 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)