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NDB ID: PR0354    PDB ID: 3EPL 


Title:

CRYSTALLOGRAPHIC SNAPSHOTS OF EUKARYOTIC DIMETHYLALLYLTRANSFERASE ACTING ON TRNA: INSIGHT INTO TRNA RECOGNITION AND REACTION MECHANISM

Molecular Description:

tRNA isopentenyltransferase (E.C.2.5.1.8)/RNA complex

Structural Keywords:

T SINGLE STRAND, HAIRPIN LOOP, LOOP

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Zhou, C., Huang, R.H.
Crystallographic snapshots of eukaryotic dimethylallyltransferase acting on tRNA: insight into tRNA recognition and reaction mechanism. 
Proc.Natl.Acad.Sci.Usa, 105, pp. 16142 - 16147, 2008.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 2 2 21

Cell Constants:

a = 164.074   b = 209.85   c = 127.318 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.0 for 20022 reflections in the resolution range 50.0 to 3.6 Ångstroms with Fobs >  1495.0 sigma(Fobs) and with I >  0.0 sigma(I)