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NDB ID: PR0379    PDB ID: 3HAX 


Title:

CRYSTAL STRUCTURE OF A SUBSTRATE-BOUND GAR1-MINUS H/ACA RNP FROM PYROCOCCUS FURIOSUS

Molecular Description:

Probable tRNA pseudouridine synthase B (E.C.5.4.99.-)
Ribosome biogenesis protein Nop10
50S ribosomal protein L7Ae/RNA complex

Structural Keywords:

DOUBLE HELIX, HAIRPIN LOOP

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 3 protein sequences

Primary Citation:

Duan, J., Li, L., Lu, J., Wang, W., Ye, K.
Structural mechanism of substrate RNA recruitment in H/ACA RNA-guided pseudouridine synthase. 
Mol.Cell, 34, pp. 427 - 439, 2009.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 2

Cell Constants:

a = 187.809   b = 63.696   c = 83.848 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC 5.2.0019 program. The R value is 0.20389 for 54667 reflections in the resolution range 20.0 to 2.11 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)