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NDB ID: PR0380    PDB ID: 3HAY 


Title:

CRYSTAL STRUCTURE OF A SUBSTRATE-BOUND FULL H/ACA RNP FROM PYROCOCCUS FURIOSUS

Molecular Description:

Probable tRNA pseudouridine synthase B (E.C.5.4.99.-)
Small nucleolar rnp gar1-like protein
Ribosome biogenesis protein Nop10
50S ribosomal protein L7Ae/RNA complex

Structural Keywords:

DOUBLE HELIX, HAIRPIN LOOP

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 4 protein sequences

Primary Citation:

Duan, J., Li, L., Lu, J., Wang, W., Ye, K.
Structural mechanism of substrate RNA recruitment in H/ACA RNA-guided pseudouridine synthase. 
Mol.Cell, 34, pp. 427 - 439, 2009.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 64 2 2

Cell Constants:

a = 189.518   b = 189.518   c = 279.045 (Ångstroms)

α = 90.0   β = 90.0   γ = 120.0 (degrees)

Refinement:

The structure was refined using the REFMAC 5.2.0019 program. The R value is 0.32494 for 12531 reflections in the resolution range 20.0 to 4.99 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)