NDB ID: RR0022 PDB ID: 1HNZ 
NAKB(beta): RR0022 
Title:
STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH HYGROMYCIN B
Molecular Description:
INHIBITED 30S RIBOSOMAL SUBUNIT
Deposited:
2000-12-08
Released:
2001-02-21
Structural Keywords:
SINGLE STRAND
Nucleic Acid Sequence:
Click to show/hide 2 nucleic acid sequences
Chain A
UUUGUUGGAGAGUUUGAUCCUGGCUCAGGGUGAACGCUGGCGGCGUGCCUAAGACAUGCAAGUCGUGCGGGCCGCGGGGU UUUACUCCG
UGGUCAGCGGCGGACGGGUGAGUAACGCGUGGGUGACCUACCCGGAAGAGGGGGACAACCCGGGGAAACUC GGGCUAAUCCCCCAUGUG
GACCCGCCCCUUGGGGUGUGUCCAAAGGGCUUUGCCCGCUUCCGGAUGGGCCCGCGUCCCAU CAGCUAGUUGGUGGGGUAAUGGCCCAC
CAAGGCGACGACGGGUAGCCGGUCUGAGAGGAUGGCCGGCCACAGGGGCACUG AGACACGGGCCCCACUCCUACGGGAGGCAGCAGUUA
GGAAUCUUCCGCAAUGGGCGCAAGCCUGACGGAGCGACGCCGCU UGGAGGAAGAAGCCCUUCGGGGUGUAAACUCCUGAACCCGGGACG
AAACCCCCGACGAGGGGACUGACGGUACCGGGGUA AUAGCGCCGGCCAACUCCGUGCCAGCAGCCGCGGUAAUACGGAGGGCGCGAGCG
UUACCCGGAUUCACUGGGCGUAAAGG GCGUGUAGGCGGCCUGGGGCGUCCCAUGUGAAAGACCACGGCUCAACCGUGGGGGAGCGUGGG
AUACGCUCAGGCUAGAC GGUGGGAGAGGGUGGUGGAAUUCCCGGAGUAGCGGUGAAAUGCGCAGAUACCGGGAGGAACGCCGAUGGCGA
AGGCAGCC ACCUGGUCCACCCGUGACGCUGAGGCGCGAAAGCGUGGGGAGCAAACCGGAUUAGAUACCCGGGUAGUCCACGCCCUAAA
CGAUGCGCGCUAGGUCUCUGGGUCUCCUGGGGGCCGAAGCUAACGCGUUAAGCGCGCCGCCUGGGGAGUACGGCCGCAAG GCUGAAACU
CAAAGGAAUUGACGGGGGCCCGCACAAGCGGUGGAGCAUGUGGUUUAAUUCGAAGCAACGCGAAGAACCUU ACCAGGCCUUGACAUGCU
AGGGAACCCGGGUGAAAGCCUGGGGUGCCCCGCGAGGGGAGCCCUAGCACAGGUGCUGCAUG GCCGUCGUCAGCUCGUGCCGUGAGGUG
UUGGGUUAAGUCCCGCAACGAGCGCAACCCCCGCCGUUAGUUGCCAGCGGUUC GGCCGGGCACUCUAACGGGACUGCCCGCGAAAGCGG
GAGGAAGGAGGGGACGACGUCUGGUCAGCAUGGCCCUUACGGCC UGGGCGACACACGUGCUACAAUGCCCACUACAAAGCGAUGCCACC
CGGCAACGGGGAGCUAAUCGCAAAAAGGUGGGCCC AGUUCGGAUUGGGGUCUGCAACCCGACCCCAUGAAGCCGGAAUCGCUAGUAAUC
GCGGAUCAGCCAUGCCGCGGUGAAUA CGUUCCCGGGCCUUGUACACACCGCCCGUCACGCCAUGGGAGCGGGCUCUACCCGAAGUCGCC
GGGAGCCUACGGGCAGG CGCCGAGGGUAGGGCCCGUGACUGGGGCGAAGUCGUAACAAGGUAGCUGUACCGGAAGGUGCGGCUGGAUCA
CCUCCUUU CU
Chain X
CUUUCU
Protein Sequence:
Click to show/hide 20 protein sequences
Chain S
MPRSLKKGVFVDDHLLEKVLELNAKGEKRLIKTWSRRSTIVPEMVGHTIAVYNGKQHVPVYITENMVGHKLGEFAPTRTY RGHGKEAKA
TKKK
Chain T
MAQKKPKRNLSALKRHRQSLKRRLRNKAKKSAIKTLSKKAVQLAQEGKAEEALKIMRKAESLIDKAAKGSTLHKNAAARR KSRLMRKVR
QLLEAAGAPLIGGGLSA
Chain V
GKGDRRTRRGKIWRGTYGKYRPRKKK
Chain B
MPVEITVKELLEAGVHFGHERKRWNPKFARYIYAERNGIHIIDLQKTMEELERTFRFIEDLAMRGGTILFVGTKKQAQDI VRMEAERAG
MPYVNQRWLGGMLTNFKTISQRVHRLEELEALFASPEIEERPKKEQVRLKHELERLQKYLSGFRLLKRLPD AIFVVDPTKEAIAVREAR
KLFIPVIALADTDSDPDLVDYIIPGNDDAIRSIQLILSRAVDLIIQARGGVVEPSPSYALVQ EAEATETPEGESEVEA
Chain D
MGRYIGPVCRLCRREGVKLYLKGERCYSPKCAMERRPYPPGQHGQKRARRPSDYAVRLREKQKLRRIYGISERQFRNLFE EASKKKGVT
GSVFLGLLESRLDNVVYRLGFAVSRRQARQLVRHGHITVNGRRVDLPSYRVRPGDEIAVAEKSRNLELIRQ NLEAMKGRKVGPWLSLDV
EGMKGKFLRLPDREDLALPVNEQLVIEFYSR
Chain C
MGNKIHPIGFRLGITRDWESRWYAGKKQYRHLLLEDQRIRGLLEKELYSAGLARVDIERAADNVAVTVHVAKPGVVIGRG GERIRVLRE
ELAKLTGKNVALNVQEVQNPNLSAPLVAQRVAEQIERRFAVRRAIKQAVQRVMESGAKGAKVIVSGRIGGA EQARTEWAAQGRVPLHTL
RANIDYGFALARTTYGVLGVKAYIFLGEVIGGQKPKARPELPKAEERPRRRRPAVRVKKEE
Chain F
MRRYEVNIVLNPNLDQSQLALEKEIIQRALENYGARVEKVEELGLRRLAYPIAKDPQGYFLWYQVEMPEDRVNDLARELR IRDNVRRVM
VVKSQEPFLANA
Chain E
MPETDFEEKMILIRRTARMQAGGRRFRFGALVVVGDRQGRVGLGFGKAPEVPLAVQKAGYYARRNMVEVPLQNGTIPHEI EVEFGASKI
VLKPAAPGTGVIAGAVPRAILELAGVTDILTKELGSRNPINIAYATMEALRQLRTKADVERLRKGEAHAQA QG
Chain H
MLTDPIADMLTRIRNATRVYKESTDVPASRFKEEILRILAREGFIKGYERVDVDGKPYLRVYLKYGPRRQGPDPRPEQVI HHIRRISKP
GRRVYVGVKEIPRVRRGLGIAILSTSKGVLTDREARKLGVGGELICEVW
Chain G
MARRRRAEVRQLQPDLVYGDVLVTAFINKIMRDGKKNLAARIFYDACKIIQEKTGQEPLKVFKQAVENVKPRMEVRSRRV GGANYQVPM
EVSPRRQQSLALRWLVQAANQRPERRAAVRIAHELMDAAEGKGGAVKKKEDVERMAEANRAYAHYRW
Chain J
MPKIRIKLRGFDHKTLDASAQKIVEAARRSGAQVSGPIPLPTRVRRFTVIRGPFKHKDSREHFELRTHNRLVDIINPNRK TIEQLMTLD
LPTGVEIEIKTVGGGR
Chain I
MEQYYGTGRRKEAVARVFLRPGNGKVTVNGQDFNEYFQGLVRAVAALEPLRAVDALGRFDAYITVRGGGKSGQIDAIKLG IARALVQYN
PDYRAKLKPLGFLTRDARVVERKKYGKHKARRAPQYSKR
Chain L
MVALPTINQLVRKGREKVRKKSKVPALKGAPFRRGVCTVVRTVTPKKPNSALRKVAKVRLTSGYEVTAYIPGEGHNLQEH SVVLIRGGR
VKDLPGVRYHIVRGVYDAAGVKDRKKSRSKYGTKKPKEAAKTAAKK
Chain K
MAKKPSKKKVKRQVASGRAYIHASYNNTIVTITDPDGNPITWSSGGVIGYKGSRKGTPYAAQLAALDAAKKAMAYGMQSV DVIVRGTGA
GREQAIRALQASGLQVKSIVDDTPVPHNGCRPKKKFRKAS
Chain N
MARKALIEKAKRTPKFKVRAYTRCVRCGRARSVYRFFGLCRICLRELAHKGQLPGVRKASW
Chain M
MARIAGVEIPRNKRVDVALTYIYGIGKARAKEALEKTGINPATRVKDLTEAEVVRLREYVENTWKLEGELRAEVAANIKR LMDIGCYRG
LRHRRGLPVRGQRTRTNARTRKGPRKTVAGKKKAPRK
Chain P
MVKIRLARFGSKHNPHYRIVVTDARRKRDGKYIEKIGYYDPRKTTPDWLKVDVERARYWLSVGAQPTDTARRLLRQAGVF RQEAREGA
Chain O
MPITKEEKQKVIQEFARFPGDTGSTEVQVALLTLRINRLSEHLKVHKKDHHSHRGLLMMVGQRRRLLRYLQREDPERYRA LIEKLGIRG
Chain R
MSTKNAKPKKEAQRRPSRKAKVKATLGEFDLRDYRNVEVLKRFLSETGKILPRRRTGLSGKEQRILAKTIKRARILGLLP FTEKLVRK
Chain Q
MPKKVLTGVVVSDKMQKTVTVLVERQFPHPLYGKVIKRSKKYLAHDPEEKYKLGDVVEIIESRPISKRKRFRVLRLVESG RMDLVEKYL
IRRQNYQSLSKRGGKA
Primary Citation:
Brodersen, D.E., Clemons Jr., W.M., Carter, A.P., Morgan-Warren, R.J., Wimberly, B.T., Ramakrishnan, V.
The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit. 
Cell(Cambridge,Mass.), 103, pp. 1143 - 1154, 2000.
Experimental Information:
X-RAY DIFFRACTION
Space Group:
P 41 21 2
Cell Constants:
a = 402.063 b = 402.063 c = 175.263 (Ångstroms)
α = 90.0 β = 90.0 γ = 90.0 (degrees)
Refinement:
The structure was refined using the CNS program.
The R value is 0.218 for 187963 reflections in
the resolution range 50.0 to 3.3 Ångstroms
with Fobs > 0.0 sigma(Fobs) and with I > 0.0 sigma(I)