NDB ID: RR0041 PDB ID: 1K01 
Title:
STRUCTURAL BASIS FOR THE INTERACTION OF ANTIBIOTICS WITH THE PEPTIDYL TRANSFERASE CENTER IN EUBACTERIA
Molecular Description:
50S RIBOSOMAL SUBUNIT WITH CHLORAMPHENICOL
Deposited:
2001-09-17
Released:
2001-10-26
Structural Keywords:
SINGLE STRAND
Nucleic Acid Sequence:
Click to show/hide 1 nucleic acid sequences
Chain A
GGUCAAGAUAGUAAGGGUCCACGGUGGAUGCCCUGGCGCUGGAGCCGAUGAAGGACGCGAUUACCUGCGAAAAGCCCCGA CGAGCUGGA
GAUACGCUUUGACUCGGGGAUGUCCGAAUGGGGAAACCCACCUCGUAAGAGGUAUCCGCAAGGAUGGGAAC UCAGGGAACUGAAACAUC
UCAGUACCUGAAGGAGAAGAAAGAGAAUUCGAUUCCGUUAGUAGCGGCGAGCGAACCCGGAU CAGCCCAAACCGAAACGCUUGCGUUUC
GGGGUUGUAGGACCAGUUUUUAAGAUUCAACCCCUCAAGCCGAAGUGGCUGGA AAGCUACACCUCAGAAGGUGAGAGUCCUGUAGGCGA
ACGAGCGGUUGACUGUACUGGCACCUGAGUAGGUCGUUGUUCGU GAAACGAUGACUGAAUCCGCGCGGACCACCGCGCAAGGCUAAAUA
CUCCCAGUGACCGAUAGCGCAUAGUACCGUGAGGG AAAGGUGAAAAGAACCCCGGGAGGGGAGUGAAAGAGAACCUGAAACCGUGGACU
UACAAGCAGUCAUGGCACCUUAUGCG UGUUAUGGCGUGCCUAUUGAAGCAUGAGCCGGCGACUUAGACCUGACGUGCGAGCUUAAGUUG
AAAAACGGAGGCGGAGC GAAAGCGAGUCCGAAUAGGGCGGCAUUAGUACGUCGGGCUAGACUCGAAACCAGGUGAGCUAAGCAUGACCA
GGUUGAAA CCCCCGUGACAGGGGGCGGAGGACCGAACCGGUGCCUGCUGAAACAGUCUCGGAUGAGUUGUGUUUAGGAGUGAAAAGCU
AACCGAACCUGGAGAUAGCUAGUUCUCCCCGAAAUGUAUUGAGGUACAGCCUCGGAUGUUGACCAUGUCCUGUAGAGCAC UCACAAGGC
UAGGGGGCCUACCAGCUUACCAAACCUUAUGAAACUCCGAAGGGGCACGCGUUUAGUCCGGGAGUGAGGCU GCGAGAGCUAACUUCCGU
AGCCGAGAGGGAAACAACCCAGACCAUCAGCUAAGGUCCCUAAAUGAUCGCUCAGUGGUUAA GGAUGUGUCGUCGCAUAGACAGCCAGG
AGGUUGGCUUAGAAGCAGCCACCCUUCAAAGAGUGCGUAAUAGCUCACUGGUC GAGUGACGAUGCGCCGAAAAUGAUCGGGGCUCAAGU
GAUCUACCGAAGCUAUGGAUUCAACUCGCGAAGCGAGUUGUCUG GUAGGGGAGCGUUCAGUCCGCGGAGAAGCCAUACCGGAAGGAGUG
GUGGAGCCGACUGAAGUGCGGAUGCCGGCAUGAGU AACGAUAAAAGAAGUGAGAAUCUUCUUCGCCGUAAGGACAAGGGUUCCUGGGGA
AGGGUCGUCCGCCCAGGGAAAGUCGG GACCUAAGGUGAGGCCGAACGGCGCAGCCGAUGGACAGCAGGUCAAGAUUCCUGCACCGAUCA
UGUGGAGUGAUGGAGGG ACGCAUUACGCUAUCCAAUGCCAAGCUAUGGCUAUGCUGGUUGGUACGCUCAAGGGCGAUCGGGUCAGAAAA
UCUACCGG UCACAUGCCUCAGACGUAUCGGGAGCUUCCUCGGAAGCGAAGUUGGAAACGCGACGGUGCCAAGAAAAGCUUCUAAACGU
UGAAACAUGAUUGCCCGUACCGCAAACCGACACAGGUGUCCGAGUGUCAAUGCACUAAGGCGCGCGAGAGAACCCUCGUU AAGGAACUU
UGCAAUCUCACCCCGUAACUUCGGAAGAAGGGGUCCCCACGCUUCGCGUGGGGCGCAGUGAAUAGGCCCAG GCGACUGUUUACCAAAAU
CACAGCACUCUGCCAACACGAACAGUGGACGUAUAGGGUGUGACGCCUGCCCGGUGCCGGAA GGUCAAGUGGAGCGGUGCAAGCUGCGA
AAUGAAGCCCCGGUGAACGGCGGCCGUAACUAUAACGGUCCUAAGGUAGCGAA AUUCCUUGUCGGGUAAGUUCCGACCUGCACGAAAGG
CGUAACGAUCUGGGCGCUGUCUCAACGAGGGACUCGGUGAAAUU GAAUUGGCUGUAAAGAUGCGGCCUACCCGUAGCAGGACGAAAAGA
CCCCGUGGAGCUUUACUAUAGUCUGGCAUUGGGAU UCGGGUUUCUCUGCGUAGGAUAGGUGGGAGCCUGCGAAACUGGCCUUUUGGGGU
CGGUGGAGGCAACGGUGAAAUACCAC CCUGAGAAACUUGGAUUUCUAACCUGAAAAAUCACUUUCGGGGACCGUGCUUGGCGGGUAGUU
UGACUGGGGCGGUCGCC UCCCAAAAUGUAACGGAGGCGCCCAAAGGUCACCUCAAGACGGUUGGAAAUCGUCUGUAGAGCGCAAAGGUA
GAAGGUGG CUUGACUGCGAGACUGACACGUCGAGCAGGGAGGAAACUCGGGCUUAGUGAACCGGUGGUACCGUGUGGAAGGGCCAUCG
AUCAACGGAUAAAAGUUACCCCGGGGAUAACAGGCUGAUCUCCCCCGAGAGUCCAUAUCGGCGGGGAGGUUUGGCACCUC GAUGUCGGC
UCGUCGCAUCCUGGGGCUGAAGAAGGUCCCAAGGGUUGGGCUGUUCGCCCAUUAAAGCGGCACGCGAGCUG GGUUCAGAACGUCGUGAG
ACAGUUCGGUCUCUAUCCGCUACGGGCGCAGGAGAAUUGAGGGGAGUUGCUCCUAGUACGAG AGGACCGGAGUGAACGGACCGCUGGUC
UCCCUGCUGUCGUACCAACGGCACAUGCAGGGUAGCUAUGUCCGGAACGGAUA ACCGCUGAAAGCAUCUAAGCGGGAAGCCAGCCCCAA
GAUGAGUUCUCCCACUGUUUAUCAGGUAAGACUCCCGGAAGACC ACCGGGUUAAGAGGCCAGGCGUGCACGCAUAGCAAUGUGUUCAGC
GGACUGGUGCUCAUCAGUCGAGGUCUUGACCACUC
Protein Sequence:
Click to show/hide 3 protein sequences
Chain L
MTAPEQTFRNKKQRKQQVKLRKPGFAVAKYVRMSPRKVRLVVDVIRGKSVQDAEDLLRFIPRSASEPVAKVLNSAKANAL HNDEMLEDR
LFVKEAYVDAGPTLKRLIPRARGSANIIKKRTSHITIIVAEKGNK
Chain K
MAQINVIGQNGGRTIELPLPEVNSGVLHEVVTWQLASRRRGTASTRTRAQVSKTGRKMYGQKGTGNARHGDRSVPTFVGG GVAFGPKPR
SYDYTLPRQVRQLGLAMAIASRQEGGKLVAVDGFDIADAKTKNFISWAKQNGLDGTEKVLLVTDDENTRRA ARNVSWVSVLPVAGVNVY
DILRHDRLVIDAAALEIVEEEAGEEQQ
Chain M
MAKHPVPKKKTSKSKRDMRRSHHALTAPNLTECPQCHGKKLSHHICPNCGYYDGRQVLAV
Primary Citation:
Schlunzen, F., Zarivach, R., Harms, J., Bashan, A., Tocilj, A., Albrecht, R., Yonath, A., Franceschi, F.
Structural basis for the interaction of antibiotics with the peptidyl transferase centre in eubacteria.
Nature, 413, pp. 814 - 821, 2001.
Experimental Information:
X-RAY DIFFRACTION
Space Group:
I 2 2 2
Cell Constants:
a = 171.1 b = 409.3 c = 696.9 (Ångstroms)
α = 90.0 β = 90.0 γ = 90.0 (degrees)
Refinement:
The structure was refined using the CNS program.
The R value is 0.281 for 238661 reflections in
the resolution range 30.0 to 3.5 Ångstroms
with Fobs > 0.0 sigma(Fobs) and with I > 0.0 sigma(I)
