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NDB ID: UR0195    PDB ID: 3GS8 


Title:

AN ALL-RNA HAIRPIN RIBOZYME A38N1DA38 VARIANT WITH A TRANSITION-STATE MIMIC SUBSTRATE STRAND

Molecular Description:

5'-R(*UP*CP*CP*CP*AP*GP*UP*CP*CP*AP*CP*CP*GP*U)-3'
2-[2-(2-HYDROXYETHOXY)ETHOXY]ETHYL DIHYDROGEN PHOSPHATE

Deposited:

2009-03-26

Released:

2009-04-21

Nucleic Acid Sequence:

Click to show/hide 4 nucleic acid sequences

Protein Sequence:

No Protein Sequence Found

Primary Citation:

Spitale, R.C., Volpini, R., Heller, M.G., Krucinska, J., Cristalli, G., Wedekind, J.E.
Identification of an imino group indispensable for cleavage by a small ribozyme. 
J.Am.Chem.Soc., 131, pp. 6093 - 6095, 2009.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 61 2 2

Cell Constants:

a = 93.82   b = 93.82   c = 134.76 (Ångstroms)

α = 90.0   β = 90.0   γ = 120.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.22 for 8402 reflections in the resolution range 27.37 to 2.85 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)