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NDB ID: pd0547    PDB ID: 1T9I 


Title:

I-CREI(D20N)/DNA COMPLEX

Molecular Description:

Deposited:

2004-05-17

Released:

2004-11-16

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Chevalier, B., Sussman, D., Otis, C., Noel, A.J., Turmel, M., Lemieux, C., Stephens, K., Monnat Jr., R.J., Stoddard, B.L.
Metal-Dependent DNA Cleavage Mechanism of the I-CreI LAGLIDADG Homing Endonuclease. 
Biochemistry, 43, pp. 14015 - 14026, 2004.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 43.8   b = 67.31   c = 89.35 (Ångstroms)

α = 90.0   β = 92.4   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.194 for 67746 reflections in the resolution range 19.97 to 1.6 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)